HLA Genotyping and Antibody Characterization Using the Luminex™ Multiplex Technology.
نویسنده
چکیده
The Luminex-based human leukocyte antigen (HLA) antibody screening technology is widespread used in laboratories affiliated to kidney transplantation programs and enables both screening (i.e. the definition of positive or negative antibody status) and antibody identification with high sensitivity and specificity. HLA typing at different levels of resolution with Luminex technology uses sequence-specific oligonucleotide probes bound to color-coded microbeads in order to identify HLA alleles encoded by the DNA sample. In general, the Luminex technology for histocompatibility analyses provides rapid sample processing in a 96-well format combined with limited technical complexity which means high cost efficiency.
منابع مشابه
Complement-dependent cytotoxicity and Luminex technology for human leucocyte antigen antibody detection in kidney transplant candidates exposed to different sensitizing events
Background The aim of this study was to determine the frequency of exposure to different sensitizing events (SEs) and to assess their effects on human leucocyte antigen (HLA) alloimmunization in transplant candidates using two different HLA antibody screening techniques: complement-dependent cytotoxicity (CDC) and Luminex. Methods This retrospective study included HLA antibody screening resul...
متن کاملPre-formed donor-specific alloantibodies (DSA) detected only by luminex technology using HLA-coated microspheres and causing acute humoral rejection and kidney graft dysfunction.
The different methodologies for the detection of HLA sensitization could have discrepant results. At the moment, luminex technology seems--in our opinion--to be the most sensitive and safest method for antibody detection, and it should be taken into account during the transplantation process and as a means of indicating immunossuppresive regimen modulation.
متن کاملAre all donor-specific antibodies detected by solid-phase assay before transplantation clinically relevant?
Sera were tested for anti-HLA antibodies using the multiplex technology SPA. Class I (i.e. HLA-A/B) and class II (i.e. HLA-DR/DP/DQ) HLA antibodies were tested using reagents from One Lambda, Inc., Canoga Park CA, (LabScreen LS1A01 Lot 007 and LS2A01 Lot 004, commercialized in Switzerland by Ingen). Briefly, 20 μl of serum samples were incubated with HLA class I and HLA class II coated microsph...
متن کاملDevelopment of internal controls for the Luminex instrument as part of a multiplex seven-analyte viral respiratory antibody profile.
The ability of the Luminex system to simultaneously quantitate multiple analytes from a single sample source has proven to be a feasible and cost-effective technology for assay development. In previous studies, my colleagues and I introduced two multiplex profiles consisting of 20 individual assays into the clinical laboratory. With the Luminex instrument's ability to classify up to 100 distinc...
متن کاملCharacterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain
BH2, a monoclonal antibody prepared against the denatured human leukocytic antigen-B27 heavy chain (HLA-B27 HC), can immunoprecipitate the misfolded HLA-B27 HC complexed with Bip in the endoplasmic reticulum and recognize the homodimerized HLA-B27 HC that is often observed on the cell membrane of patients suffered from ankylosing spondylitis (AS). However, the recognition specificity of BH2 tow...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie
دوره 36 4 شماره
صفحات -
تاریخ انتشار 2009